2017-8-11 16:0:32025-05-30 14:11:04
description
sporulation-specific penicillin-binding protein 4b
[SW|sporulation]-specific class B penicillin-binding protein 4b
locus
BSU27310
BSU_27310
geneLength
1752
1755
ec
outlinks
bsu
BSU27310
BSU_27310
Gene
Coordinates
2,789,686 → 2,791,440
2,789,686 2,791,440
The protein
Protein family
transpeptidase family (according to Swiss-Prot)
[SW|transpeptidase family] (according to UniProt)
References
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3OC2] (PBP3 from Pseudomonas aeruginosa, 23% identity) [pubmed|20974151]
The protein
[SW|Localization]
anchored to the membrane, the major part is exposed to the outside (according to UniProt)
during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
Biological materials
Mutant
BKE27310 ([[gene|pbpI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA
BKK27310 ([[gene|pbpI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA
Biological materials
GFP fusion
2081 [[gene|trpC]]2 [[gene|pbpI]]::pSG1489 (cat Pxyl-gfpa-[[gene|pbpI]]1-430) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]