SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-30 14:11:04

description

sporulation-specific penicillin-binding protein 4b

[SW|sporulation]-specific class B penicillin-binding protein 4b

locus

BSU27310

BSU_27310

geneLength

1752

1755

ec

outlinks

bsu

BSU27310

BSU_27310

Gene

Coordinates

2,789,686 → 2,791,440

2,789,686 2,791,440

The protein

Protein family

transpeptidase family (according to Swiss-Prot)

[SW|transpeptidase family] (according to UniProt)

References

14679248, 15699190, 16497325, 15758244

14679248, 15699190, 16497325, 15758244, 14731276, 20974151, 30651563

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|3OC2] (PBP3 from Pseudomonas aeruginosa, 23% identity) [pubmed|20974151]

The protein

[SW|Localization]

anchored to the membrane, the major part is exposed to the outside (according to UniProt)

during vegetative growth: evenly distributed along the membrane [pubmed|14731276]

Biological materials

Mutant

BKE27310 ([[gene|pbpI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA

BKK27310 ([[gene|pbpI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA

Biological materials

GFP fusion

2081 [[gene|trpC]]2 [[gene|pbpI]]::pSG1489 (cat Pxyl-gfpa-[[gene|pbpI]]1-430) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]